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Image Search Results
Journal: BMC Cancer
Article Title: Targeting tissue factor on tumour cells and angiogenic vascular endothelial cells by factor VII-targeted verteporfin photodynamic therapy for breast cancer in vitro and in vivo in mice
doi: 10.1186/1471-2407-10-235
Figure Lengend Snippet: TF is expressed on tumour cells and VEGF-stimulated HUVECs but not on unstimulated HUVECs . A . TF expression was detected on breast cancer cell lines but not on CHO-K1 cells by flow cytometry using mouse Icon (mfVII/hIgG1 Fc) protein. B . Selective expression of TF on VEGF-stimulated HUVECs but not on unstimulated HUVECs was observed and photographed under confocal microscope. HUVECs were stained for TF expression by mouse Icon or anti-HTF antibody (FITC, green) and then by anti-human CD31 PE (red) to verify that TF-expressing cells were of vascular endothelial origin. Results in A and B are representative of two experiments.
Article Snippet:
Techniques: Expressing, Flow Cytometry, Microscopy, Staining
Journal: BMC Cancer
Article Title: Targeting tissue factor on tumour cells and angiogenic vascular endothelial cells by factor VII-targeted verteporfin photodynamic therapy for breast cancer in vitro and in vivo in mice
doi: 10.1186/1471-2407-10-235
Figure Lengend Snippet: fVII targeting improves the selectivity of VP PDT . A . Incubation time of fVII-VP and free VP affected the effect of fVII-tPDT and ntPDT (2 μM, 689 nm laser at 60 J/cm 2 ) on killing MDA-MB-231 cancer cells. However, VP alone without laser irradiation (VP alone) did not have an effect on killing the cancer cells. B . Comparison of fVII-tPDT and ntPDT (689 nm laser at 60 J/cm 2 ) for side effects on non-TF-expressing CHO-K1 cells as a normal cell line. C . VP PDT (fVII-tPDT or ntPDT with 0.5 or 5 μM VP at 60 J/cm 2 ) for the non-TF expressing 293 line as a normal cell line control. D and E . fVII-tPDT and ntPDT (689 nm laser at 36 J/cm 2 ) for HUVEC with or without induction of TF expression by VEGF, as seen in Figure 2B. Results in A-D are representative of two experiments.
Article Snippet:
Techniques: Incubation, Irradiation, Comparison, Expressing, Control